The AquaBoost® range of products from Cryogenetics are carefully designed to maximize the potential of sperm and eggs from fish. The series consist of several unique products that can be used individually or in conjunction as part of a fertilization workflow.
Specially designed for zebrafish (Danio rerio), AquaBoost® OvaCoat is a holding medium for eggs after they are stripped from the female for in vitro fertilization (IVF) use. Zebrafish eggs are very fragile and egg quality rapidly deteriorates after stripping, often becoming unsuitable for IVF after just 5 minutes.
By applying OvaCoat, holding time can be increased significantly (up to 30 minutes) without a reduction in fertilization results. Therefore time constraints becomes less critical to a successful IVF result and leads to a better workflow. Eggs treated with AquaBoost® OvaCoat have been held for more than 30 minutes after stripping with no significant reduction in quality.
The use of AquaBoost® OvaCoat is recommended for IVF with both fresh and cryopreserved sperm. The product is easy to use and comes in small batches suitable for multiple applications.
You can download more information about AquaBoost® OvaCoat here (pdf).