Cryopreservation is the fundamentally storage of genetic materials for reuse at a later date. Cryopreservation is storage at very low temperatures to stop decay in the materials and maintain them in a stable condition. When cryopreserving living cells for rejuvenation at a point in the future the techniques are much more complicated and success rate of future cell viability can vary enormously.
We at Cryogenetics have developed a whole range of cryopreservation media and protocols that offer predictable, high fertilization rates of milt that has been stored in liquid nitrogen at -196oC and then thawed in a controlled way.
How is this done?
You the client, after discussing your exact requirements with us, will either request that we strip the milt for you on your site or you do this yourself and send the milt to one of our laboratories in chilled containers.
Upon receipt of the milt we ensure that the shipment is suitably disinfected before entering our “clean rooms” which are kept between 2-4oC at all times.
Sample from each male will be checked and tested (See: Concentration & Quality Measurement) and then the milt will be diluted to maximize the fertility benefit that the sperm cells can achieve, or higher egg to sperm ratio.
The sperm is now in the optimal condition for cryopreservation and it is transferred, based on quantity, to either straws or SquarePack®, then sealed and approved for storage into liquid nitrogen.
Some useful numbers (in terms of salmon)
1 Straw contains 0,5 ml of milt – enough to fertilize 150 eggs
1 SquarePack® contains 12,5 ml of milt – enough to fertilize 3,000 – 4,000 eggs