Concentration & quality measurement

The large variation in sperm concentration between males and ejaculates is well documented. Therefore, when a specific amount of milt routinely is used for fertilization, this leads to reduced fertilization efficiency and lost production potential. Knowing the sperm concentration in each ejaculate enables the user to optimize the sperm to egg ratio and to maximize the number of fertilized eggs produced. Cryogenetics has for several years used photometers to measure sperm concentration and has solid, evidence-based know-how in this field for multiple fish species.

With the SDM6 Photometer you can measure the sperm concentration in your own facility

The SDM6 Photometer runs precise measurement of sperm concentration, and has several advantages:
• Rapid measurement of sperm concentration – less than 1 minute per sample
• Integrated printer for recording data
• Low cost for analyzed samples – less than 0.2 Euro (30 cents) per sample
• Robust instrument requiring little to no maintenance

As sperm concentration data is analyzed, a medium is needed to dilute the original ejaculate in order to obtain a standardized sperm to egg ratio. When the volume required to fertilize a batch of eggs is reduced, a dilutor will add volume, making it easier to distribute the sperms cells to cover all of the eggs before activation.

Cryogenetics has developed the AquaBoost® series of products to facilitate these needs.

The precise measurement of sperm concentration in an SDM6 Photometer provides you with consistently reliable calculations for the amount of AquaBoost® Dilutor or AquaBoost® Extender needed to maximize the fertilization potential.

Quality measurement in our lab

Upon receiving the milt at a Cryogenetics facility we evaluate its fertility potential. In this way, we can preserve the milt at optimal dilution to achieve the best fertilization possible at some time in the future as per the clients’ production plan.

On receipt of the milt, each sample and each male is given a unique identifier and logged on the database, and then tested and scored as follows:

  • Visual
    • The milt is examined for extraneous materials, such as blood or other contaminants.
  • Motility
    • A small sample is activated and examined under a microscope for its activity level and scored accordingly, high to low.
  • Concentration
    • Using our SDM6 Photometer, sperm concentration is measured, which allows us then to dilute the milt to the optimal level for cryopreservation.

More advanced quality parameters are available on clients request:

    • Extended motility analysis – CASA
    • Sperm viability – Flow cytometry
    • Sperm DNA integrity – Flow cytometry
    • IVF control with cryopreserved sperm